Oloa

Biotransformation o le methyl-β-D-glucopyranoside i filifili maualuga alkyl glucosides e sela fusifusia β-glucosidase o Pichia etchellsii.

I le suʻesuʻega nei, ua matou suʻesuʻeina le faʻaogaina o le Pichia etchellsii sela atoa mo le faʻapipiʻiina o alkyl glucosides umi.Methyl-β-d-glucopyranoside (MG) na faʻaaogaina i le tali atu i gaʻo gaʻo, n-hexanol, n-octanol, n-decanol ma le n-dodecanol e faʻapipiʻi ai le alkyl glucosides taʻitasi.O tulaga muamua o le tali na muamua fa'amauina ile fua ole 2.5 ml mo le fa'apipi'iina ole octyl glucoside (OG) ma e 8% vai, 100mM MG ma le 6h ole taimi fa'aalia ma maua ai le ≈ 53% fua.O le maualuga o le transglucosylation/hydrolysis ratio o le 2.79 na maua i le 100mM MG e fiafia i le maualuga o fua o oloa.E tusa ai ma tulaga sili ona lelei, na faʻaogaina se faʻamalo i le 50 ml maualuga lea na maua ai le ≈ 60% liua o le MG i le OG.O se auala faigofie e fa'atino ai le chromatography vai na fa'atupuina mo le fa'atusatusaina o filifili maualuga glucosides e fa'aaoga ai se su'e fa'asino igoa.Ole maualuga ole 27% ma le 13% fua na maua mo le decyl-, ma le dodecyl-β-d-glucopyranoside, faasologa.