Oloa

Ole su'esu'ega ole proteome-wide phosphorylation mea o lo'o avea pea ma lu'itau au'ili'ili ona o le lavelave tele o feso'ota'iga phosphorylation protein.I lenei galuega, matou te iloiloina le fetaui lelei o Lys-N, Lys-C, ma trypsin e tusa ai ma lo latou gafatia e saofagā i le suʻesuʻeina o le lalolagi atoa o le phosphoproteome.O se fa'ata'ita'iga fa'alelei o le fa'aliliuina o kation malosi maualalo-pH na fa'aaogaina e tu'u'ese'ese lelei ai le N-terminally acetylated, phosphorylated, ma nonmodified peptides.Ole aofaiga ole 5036 nonredundant phosphopeptides e mafai ona faʻamaonia ile fua ole suʻesuʻeina ole <1% mai le 1 mg ole polotini e faʻaaoga ai se tuufaatasiga o enzymes e tolu.O a matou faʻamatalaga na faʻaalia ai o le faʻaogaina i le va o faʻamaumauga o faʻamaumauga phosphopeptide na faʻatupuina ma protease eseese e faʻapitoa, ae o le faʻaogaina i le va o faʻamatalaga faʻamatalaga tryptic tutusa e lua na maua e le itiiti ifo i le 4 taimi maualuga.I lenei auala, o le faʻaogaina tutusa o Lys-N ma trypsin na mafai ai ona faʻateleina le 72% i le numera o phosphopeptides na maua pe a faʻatusatusa i le trypsin naʻo ia, ae o le faʻataʻitaʻiga faʻataʻitaʻiga o le trypsin na oʻo atu i le 25% faʻateleina.O le mea lea, pe a taulaʻi naʻo le trypsin ma le Lys-N faʻamaumauga, matou te iloa 4671 nonredundant phosphopeptides.O isi suʻesuʻega o nofoaga na maua na faʻaalia ai o le Lys-N ma le trypsin faʻamaumauga faʻamaumauga na faʻatamaoaigaina i le tele o faʻasologa o le phosphorylation, ma faʻamaonia atili ai o auala multiprotease e taua tele i suʻesuʻega phosphoproteome.