Oloa

O se tasi o mea e puipuia ai le faʻaaogaina o vailaʻau faʻafouga i faʻamaʻi faʻamaʻi faʻaleagaina o se puna talafeagai o sela.Chondrocytes, na o le pau lava le ituaiga sela o cartilage, tupu i vitro i lalo o tulaga faaleaganuu e faalautele ai le numera o le cell leiloa lo latou phenotype faatasi ai ma le gafatia e gaosia hyaline cartilaginous tissue.I lenei suʻesuʻega matou te iloa ai o se faʻaogaina o le tolu-dimensional (3D) aganuʻu serum-ma le tuputupu ae-factor-free toe faʻafoʻisia le gafatia o chondrocytes pasia e fausia ai le cartilage tissue in vitro, o se faʻagasologa e aofia ai sox9.Bovine articular chondrocytes na pasia faalua i. fa'ataga mo le fa'alauteleina o le numera o le sela (P2) ma fa'atupuina i le maualuga maualuga i luga ole 3D collagen-type-II-colated membranes ile fa'asalalauga maualuga ole kulukose fa'aopoopo ile inisalini ma le dexamethasone (SF3D).O sela na faʻaalia ina ua maeʻa le faʻalauteleina o le monolayer ma mulimulitaʻia le aganuu 3D e ala i le tafe o cytometry, faʻamatalaga o kene, ma tala faʻasolopito.O suiga muamua i le faʻailogaina o auala faʻaliliuina i le taimi o le reddifferentiat ion na faʻaalia. O sela P2 na faʻaalia ai se faʻamatalaga antigen e pei o tuaa o le 99% CD44 (+) ma le 40% CD105 (+) ma se faʻamatalaga faʻamatalaga faʻamatalaga faʻaalia o sela interzone.P2 i le SF3D faʻaalia chondrogenic genes ma faʻaputuina matrix extracellular.Fa'alaloina le fa'aogaina o le inisalini (IR) fa'atasi ma le HNMPA-(AM3) po'o le ala PI-3/AKT kinase (fa'agaoioia e le inisalini togafitiga) fa'atasi ai ma Wortmannin fa'asaina le fa'aogaina o le collagen.HNMPA-(AM3) faʻaitiitia faʻamatalaga o Col2, Col11, ma IR genes faʻapea foʻi ma Sox6 ma -9.Co-immunoprecipitation ma chromatin immunoprecipitation analysis o sela HNMPA-(AM3)-togafitiga na faʻaalia ai le fusia o le coactivators Sox6 ma Med12 ma Sox9 ae faʻaititia le Sox9-Col2a1 binding. Matou te faʻamatalaina se auala faʻaleaganuʻu fou e mafai ai ona faʻateleina le numera o chondrocytes ma faʻaleleia. e pei o le hyaline le fa'atupuina o aano o le cartilage i se vaega e ala i le fusifusia o le inisalini-mediated Sox9-Col2a1.E tatau ona su'esu'e i vivo le talafeagai o le aano o lo'o gaosia e ala i lenei faiga mo le fa'aogaina i le toe fa'aleleia fa'atasi.