Oloa

O le Tet 5-methylcytosine dioxygenases e faʻamalosia le DNA demethylation e ala i le gaosia o le 5-hydroxymethylcytosine ma isi oloa faʻamaʻi.Tet1 ma Tet2 o loʻo faʻaalia maualuga i sela pluripotent isumu ma faʻaititia i vaega eseese i sela somatic, ae o le faʻaogaina o auala e le o manino.O iinei na matou faʻamalamalamaina ai le faʻalauiloaina ma le faʻaleleia o vaega i Tet1 ma Tet2.I totonu o le 15-kb "superenhancer" o Tet1, e lua nofoaga amata fa'aliliuga (TSSs) fa'atasi ai ma mamanu fa'agaoioi eseese a'o atina'e.O le 6-kb promoter region i luga a'e o le TSS mamao o lo'o galue malosi i sela pluripotent naive, o lo'o ta'u sa'oloto le fa'aaliga Tet1 i se faiga fa'atransgenic, ma fa'avave ona maua le DNA methylation ma le fa'amuta i luga o le eseesega i sela fa'aleaganu'u ma epiblast moni.O le TSS lona lua i lalo, e fesoʻotaʻi ma se faʻaupuga vaivai CpG-oaoa faʻalauiloa, e faʻagaoioia e se faʻaleleia tuaoi i sela faʻamaʻi embryonic naive (ESCs) ma primed epiblast-pei sela (EpiLCs).Tet2 ei ai le CpG island p romoter ma le pluripotency-tuto'atasi gaoioiga ma se ESC-fa'apitoa fa'atupu intragenic fa'aleleia;o le mea mulimuli e vave ona faʻalaloina i EpiLCs.O a matou suʻesuʻega o loʻo faʻaalia ai auala maʻoti o tulafono faatonutonu i Tet1 ma Tet2 i le taimi o suiga o le setete o le amataga o le atinaʻe.O tusitala fou transgenic o loʻo faʻaogaina le Tet1 ma le Tet2 cis-regulatory domains e mafai ona faʻamaonia suiga faʻanumeraina i setete pluripotent ma suiga epigenetic faʻavae.