Oloa

Zwitterionic butanesulfonic acids fou e fa'alautele ai le alkaline range o aiga e fa o Pa'u Lelei: iloiloga mo le fa'aogaina i faiga fa'aola.

E fa fa'agata fa'agata fa'akomepiuta fou zwitterionic butanesulfonic e feso'ota'i fa'atulagaina i aiga e fa o Pa'u Lelei na iloiloina mo le fa'aogaina i faiga fa'aola.O nei paʻu, faʻatasi ai ma tau pKa mai le 7.6 i le 10.7, na faʻatusatusa i le tele o isi paʻu mai le aiga lava e tasi ma faʻatasi ai ma paʻu e le fesoʻotaʻi e iloa ai lo latou aʻafiaga i le gaioiga o le enzyme ma luga o le tuputupu aʻe o microbial.O le gaioiga o enzymes e fa ma tulaga maualuga o le pH i le alkaline range na tofotofoina: beta-galactosidase, esterase, phosphodiesterase ma alkaline phosphatase.I se tulaga lautele, o le lelei uma buffers, e aofia ai le butanesulfonic acid buffers fou, tuuina atu gaoioiga lelei;ae ui i lea, sa i ai suiga i le gaioiga o nisi enzymes ma ni paʻu faapitoa.Tris, glycine, ma phosphate buffers e masani ona faʻaalia suiga i gaioiga faʻatusatusa i le aiga o Good buffers.beta-Galactosidase, aemaise lava, na faʻaalia ai le tele o gaioiga faʻatasi ma Paʻu Lelei nai lo faʻamaʻi phosphate poʻo Tris.E faapena foi, o le tuputupu ae o siama siama e fitu sa tumau, faatasi ai ma ni nai tuusaunoaga, mo aiga lelei uma o paʻu ma Tris e masani ona taofia le tuputupu ae.O le fa'avasegaina o le alkaline phosphatase fa'atasi i fa'ama'i ose meafaigaluega taua i le tele o fa'aoga i le biology mole.O le tele o puipuiga lelei na maua ai fa'ailoga lelei pe a fa'atusatusa i le Tris ile pH 9.5 mo le su'esu'eina o polotini e fa'aaoga ai le alkaline phosphatase-conjugated antibodies.