Oloa

O le mataʻituina o vailaʻau faʻapitoa e faʻaopoopo i pharmacokinetics o se tasi lea o taʻiala e faʻapitoa ai le togafitiga o le mycophenolate mofetil.O le fa'amoemoega o lenei su'esu'ega o le atina'eina lea o auala ma'ale'ale chromatography-tandem mass spectrometry (LC-MS/MS) mo le iloiloga o vaila'au ma vaila'au o le mycophenolic acid (MPA).Fa'atonuga o le mycophenolic acid glucuronide (MPAG), mycophenolic acid acyl-glucuronide, faʻapea foʻi ma le MPA ma le MPAG, na faʻamoemoeina, ma o le inosine-5'-monophosphate dehydrogenase gaioiga na fuafuaina e ala i le fuaina o le maualuga o le xanthosine-5'-monophosphate (XMP) gaosia. ) ma le intracellular adenosine-5'-monophosphate pe a uma ona faʻapipiʻiina lysates toto mononuclear cell peripheral (PBMC).O se koluma Mastro(TM) e leai se u'amea ma fa'asologa gradient e lua na fa'aogaina e fa'aleleia ai le fa'atapula'aina o le XMP i le 0.1 μM.I totonu ole vaega ole fa'aogaina ole MPA ile falema'i, ole laina ole laina fa'avasegaina, va'aiga ma totonu ole aso sa'o ma le sa'o na fa'amalieina ai ta'iala talafeagai a le US Food and Drug Administration.O faʻatonuga o le MPA i le hematopoietic stem cell transplant (HSCT) maʻi na fuafuaina e le enzyme assay ma le LC-MS / MS o loʻo iai nei na faʻaalia ai se faʻamaopoopo lelei (r (2) = 0.95, p <0.001).I lenei suʻesuʻega, matou te lipotia maʻaleʻale ma faʻamaonia LC-MS / MS metotia e iloilo ai le pharmacokinetics ma pharmacodynamics o le MPA, lea e lava le maaleale e iloilo ai ni vaega laiti o le PBMC lysates na aoina i le taimi lava e uma ai le HSCT.