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Oloa

4-Nitrophenyl Beta-D-glucuronide CAS:10344-94-2 Samasama Malamalama i Paʻepaʻe Paʻu 98%

Fa'amatalaga Puupuu:

Numera fa'amaumauga: XD90021
CAS: 10344-94-2
Fuafua mole: C12H13NO9
Molecular Weight: 315.23
Avanoa: E tele
Tau:
mua'i afifi: 1g USD20
Vaega Tele: Talosaga Sesii

Fa'amatalaga Oloa

Faailoga o oloa

Numera Fa'amaumauga XD90021
Igoa Oloa 4-Nitrophenyl Beta-D-glucuronide
CAS 10344-94-2
Fuafua mole C12H13NO9
Molecular Weight 315.23
Fa'amatalaga Teuina -15 i le -20 °C
Fa'atonu Fa'asologa o Fa'asologa 29329900

Fa'amatalaga o oloa

Mama (HPLC) ≥98%
Fa'aaliga Samasama mama i le pa'epa'e pa'epa'e
HNMP E tusa ma le fausaga
Kart Fischer maualuga 10%

O se kenera beta-glucosidase (bgl3) mai Streptomyces sp.QM-B814 (American Type Culture Collection 11238) ua faʻapipiʻiina e ala i le faʻaogaina o le beta-glucosidase-negative mutant o Streptomyces lividans.O se faavaa faitau matala o 1440 nucleotides o loʻo faʻapipiʻiina se polypeptide o 479 amino acids na maua e ala i le faʻasologa.O le polotini fa'ailoga (Bgl3) o lo'o fa'aalia ai le tutusa tele (sili atu i le 45% fa'asinomaga) ma beta-glycosidases mai le aiga-1 glycosyl hydrolases.O le enzyme cloned, fa'amamāina pe a mae'a le ammonium sulphate precipitation ma lua chromatographic step, o le monomeric ma le mole mole 52.6 kDa, e pei ona fuafuaina e le tele spectrometry, ma le isoelectric point o pI 4.4.O le enzyme e foliga mai o se beta-glucosidase faʻatasi ai ma le lautele o mea e faʻapitoa, o loʻo galue i cellooligomers, ma faʻatino gaioiga transglycosylation.Ole fua fa'atatau Km mo p-nitrophenyl-beta-D-glucopyranoside ma cellobiose e 0.27 mM ma le 7.9 mM.O tau Ki mo le kulukose ma le delta-gluconolactone, faʻaaoga p-nitrophenyl-beta-D-glucopyranoside e fai ma sui, e 65 mM ma le 0.08 mM, i le faasologa.O le enzyme fa'amamaina e iai le pH sili ona maualuga o le pH 6.5 ma o le vevela sili ona lelei mo gaioiga e 50 tikeri


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    4-Nitrophenyl Beta-D-glucuronide CAS:10344-94-2 Samasama Malamalama i Paʻepaʻe Paʻu 98%