Oloa

1.Optically active D-2-hydroxybutanoate o se poloka fale taua tele mo vailaʻau ma biodegradable poly(2-hydroxybutanoate).Kinetic resolution o racemic 2-hydroxybutanoate atonu o se mea lanu meamata ma manaʻomia isi mo le gaosiga D-2-hydroxybutanoate.I lenei galuega, D-2-hydroxybutanoate i se maualuga maualuga (0.197 M) ma se maualuga enantiomeric sili atu (99.1%) na gaosia e le NAD-tutoatasi L-lactate dehydrogenase (L-iLDH) o loʻo i ai le biocatalyst.2-Oxobutanoate, o se isi mea taua tele, na faʻapipiʻiina i se maualuga maualuga (0.193 M).Faʻaaogaina o se faʻaogaina faigofie o le faʻaogaina o le ion exchange resin D301, D-2-hydroxybutanoate na vavae ese mai le biotransformation system ma le maualuga o le toe faʻaleleia o le 84.7%.

2. Asymmetric synthesis o se amino acid e le masani ai na faʻaalia e omega-transaminase mai Vibrio fluvialis JS17.L-2-Aminobutyric acid na faʻapipiʻiina mai le 2-oxobutyric acid ma le benzylamine faʻatasi ai ma se enantiomeric sili atu maualuga atu nai lo le 99%.O le tali na fa'aalia ai le malosi o le fa'asaina o oloa e le benzaldehyde, lea na fa'ato'ilaloina e ala i le fa'aogaina o se faiga fa'afoliga biphasic e aveese ai le oloa fa'asaina mai le vaega vai.I se tali masani biphasic (50 mM 2-oxobutyric acid, 70 mM benzylamine ma 2.64 U / ml faʻamamaina enzyme) faʻaaogaina le hexane e avea o se extractant, o le liua o le 2-oxobutyric acid na oʻo i le 96% i le 5 h ae na o le 39% liua na maua e aunoa ma o le mauaina o oloa.