Tris maleate Cas:72200-76-1 99.5% Paʻepaʻe tioata paʻepaʻe
Numera Fa'amaumauga | XD90057 |
Igoa Oloa | Tris maleate |
CAS | 72200-76-1 |
Fuafua mole | C4H11NO3 · C4H4O4 |
Molecular Weight | 237.21 |
Fa'amatalaga Teuina | Ambient |
Fa'atonu Fa'asologa o Fa'asologa | 29221985 |
Fa'amatalaga o oloa
Fa'aaliga | Pa'epa'e tioata pa'epa'e |
Su'ega | ≥ 99.5% |
Cocrystallization o le lysyl-tRNA synthetase mai Thermus thermophilus faatasi ai ma ona tRNAlys cognate ma Escherichia coli tRNAlys.
Lysyl-tRNA synthetase mai Thermus thermophilus ua cocrystallized a le o lona cognate tRNAlys poʻo Escherichia coli tRNAlys faʻaaogaina le ammonium sulfate e fai ma faʻafefe.O tioata e ola mai vaifofo o loʻo i ai se 1: 2.5 stoichiometry o le synthetase dimer i le tRNA i le 18-22% ammonium sulfate i le 50 mM Tris-maleate paʻu ile pH 7.5.O fa'alavelave uma e lua e fai ma sikuea prismatic, tioata tetragonal e tutusa lelei le vaega o le cell cell parameters (a = b = 233 A, c = 119 A) ma fa'aeseese i le itiiti ifo i le 2.7 A iugafono.Ae peita'i o le homocomplex o le va'aiga vaega P42(1)2 ma le heterocomplex ole va'ava'ai vaega I422.