TMB Cas:54827-17-7 99% Paʻepaʻe, paʻepaʻe paʻepaʻe i le efuefu poʻo samasama paʻu
Numera Fa'amaumauga | XD90163 |
Igoa Oloa | TMB |
CAS | 54827-17-7 |
Fuafua mole | C16H20N2 |
Molecular Weight | 240.34 |
Fa'amatalaga Teuina | 2 i le 8 °C |
Fa'atonu Fa'asologa o Fa'asologa | 29215990 |
Fa'amatalaga o oloa
Fa'aaliga | Pa'epa'e, pa'epa'e i le efuefu po'o le pa'u samasama |
Asay | 99% |
Ma'imau ile Fa'amago | <2.0% |
Mo na'o su'esu'ega fa'aoga, ae le mo le fa'aogaina e tagata | fa'aoga su'esu'e, ae le mo le fa'aogaina e tagata |
Meatotino: 3,3',5,5'-Tetramethylbenzidine o le paʻepaʻe tioata paʻepaʻe, e leai se manogi, leai se tofo, e le mafai ona faʻafefe i le vai, faigofie ona solu i le acetone, ether, dimethyl sulfoxide, dimethylformamide, ma isi mea faʻapipiʻi.
Sauniuniga: 3,3,5,5-Tetramethylbenzidine o se chromogen reagent taua.O le faʻaaogaina o le 2,6-dimethylaniline e pei o mea mata, e ala i le faʻagaoioia, fesoʻotaʻiga faʻamaʻi ma le faʻamamaina, e maua ai le 3,3,5,5-tetramethylbenzidine mama, ma o le aofaʻi atoa e oʻo atu i le 65%.
Gaioiga fa'aolaola: TMB (BMblue) ose chromogenic substrate mo immunohistochemistry ma ELISA.
Fa'aaogāina: O lenei oloa o se sui e le fa'ama'i mo le benzidine 1 (su'ega a Ames e le lelei), e talafeagai e fai ma sui o le peroxidase mo su'esu'ega immunosorbent e feso'ota'i i enzyme.O le mea'ai e maua mai ai se oloa fa'ai'uga lanu moana e mafai ona faitau fa'apitoa ile 370 po'o le 620-650 nm.Ole tali ole TMB e mafai ona taofi ile 2MH2SO4 (liliu samasama) ma faitau ile 50 nm ile 4Chemicalbook.O se mea e maʻaleʻale ma faʻapitoa mo le suʻeina o le toto, fuaina o le hemoglobin, ma le fuaina o le peroxidase.
Fa'aoga: Ose mea fou ma le saogalemu chromogen reagent;TMB ua faasolosolo ona suia le malosi o le carcinogen benzidine ma isi vailaʻau benzidine carcinogenic, ma faʻaaogaina i suʻega falemaʻi, suʻega faʻapitoa, suʻesuʻega solitulafono ma le mataʻituina o le siosiomaga ma isi matāʻupu;aemaise ile I su'esu'ega fa'aolaola ole falema'i, TMB, e fai ma sui fou mo peroxidase, ua fa'aaogaina lautele ile enzyme immunoassay (EIA) ma le enzyme-linked immunosorbent assay (ELISA);e masani ona fa'aaogaina i galuega nei: fa'ailoga fa'ailoga tamatamai lima o le toto;Vave ona iloa le ava malosi i le faua;tapenaga o fasi pepa su'e urine;fa'ama'i fa'ama'i hepatitis;su'ega e iloa ai le maitaga;fuafuaina vave o le kulukose, hemoglobin, albumin i le toto ma le urine;su'ega toto faataulaitu fecal;fuafuaina o le taua o le granulocyte i le toto, steroids, Suʻesuʻeina o hormones feusuaiga;fuafuaina o gaoioiga enzymatic;su'esu'eina o antigens, antibodies ma mea tau kenera