Oloa

O le faʻasaina o le β-carbonic anhydrases (CAs, EC 4.2.1.1) mai le pathogenic fungi Cryptococcus neoformans (Can2) ma Candida albicans (Nce103) faʻatasi ai ma se faasologa o 25 branched aliphatic ma aromatic carboxylates ua suʻesuʻeina.O tagata isoforms hCA I ma II na aofia ai foi i le suʻesuʻega mo faʻatusatusaga.Aliphatic carboxylates e masani lava o milimolar hCA I ma II inhibitors ma maualalo micromolar / submicromolar β-CA iniseti.Aromatic carboxylates o micromolar inhibitors o enzymes e fa ae o nisi oi latou na faʻaalia le maualalo o le nanomolar gaioiga e faasaga i enzymes pathogenic fungal.4-Hydroxy- ma le 4-methoxy-benzoate fa'asaina Can2 ma K(I)s o le 9.5-9.9 nM.O le methyl esters, hydroxamates, hydrazides ma carboxamides o nisi o nei mea e maua mai ai o ni inhibitors aoga foi o le α- ma le β-CA na suʻesuʻeina iinei.

O Parabens o se tasi lea o mea fa'asao e masani ona fa'aaogaina e taofia ai le tuputupu a'e o microbial ma fa'alautele ai le ola fa'aola o le tele o oloa fa'atau.O le fa'amoemoe o le su'esu'ega o lo'o iai nei o le maua lea o le fa'amatalaga i le metabolism o parabens i sela o le kanesa o le susu (MCF7) talu ai na fa'aalia le gaioiga o le estrogenic i nei sela ma ua iloa i totonu o le kanesa o le susu.O le oona o parabens i sela MCF7 na fuafuaina e faʻaaoga ai suʻega MTT.Hydrolysis o methyl-, butyl ma benzyl-paraben i le p-hydroxybenzoic acid sa auiliiliina i sela MCF7 aganuu ma i homogenates feaveai.Glucuronidation ma sulfoconjugation na suʻesuʻeina i MCF7 homogenates, ma parabens na suʻesuʻeina e le HPLC.O le Methyl-paraben na faʻaalia e matua itiiti lava le oona nai lo le butyl ma le benzyl-paraben.Parabens sa mautu atoatoa i MCF7 homogenates ae o p-nitrophenyl acetate, o se ituaiga substrate, na faia le hydrolysis.MCF7 cell homogenates e leʻi faʻaalia le glucuronidation ma le sulfoconjugation gaioiga agai i parabens.O le maualuga o le mautu o parabens e mafai ona fa'amatalaina lo latou fa'aputuina i aano o le kanesa o le susu.