Oloa

Monogalactopyranosides o le fluorescein ma le fluorescein methyl ester: faʻapipiʻi, faʻamaʻi faʻamaʻi e le biotnylated β-galactosidase, ma le faʻamautuina o le faʻasalalauga faʻasalalauga faʻatasi

Fluorescein monoglycosides (d-galactopyranoside (FMG) ma le d-glucopyranoside) ma latou methyl ester (MFMG) ua saunia mai le acetobromoglucose / galactose ma le fluorescein methyl ester i fua lelei.O fa'ata'ita'iga fa'ata'ita'iga o le fa'au'uga o le enzymatic (fa'aaogāina o le biotinylated β-galactosidase) o mea e maua mai i le galacto ua mae'a fa'atinoina ma fa'atatau i le kinetic parameters.O le 15-20 taimi fa'atuputeleina o le malosi o le fluorescence na matauina i le taimi o le hydrolysis.O se fa'atuputeleina o le fluorescence ua maitauina i se taimi pu'upu'u ma le maualalo o le fa'aogaina o mea'ai, ma fa'aogaina ai nei fa'afefiloiga aoga ma ma'ale'ale su'esu'ega mo galactosidases.Ole maualuga ole tau ole Michaelis-Menten (Km) mo le MFMG e maualuga atu nai lo le FMG o loʻo fautua mai ai se suiga faʻatatau ole fluorogenic substrate.Km tau mo le biotinylated β-Gal ma FMG e maualalo ifo nai lo le enzyme masani.O lenei fa'amatalaga e fa'ailoa ai le maualuga o le so'oga o le enzyme biotinylated pe a fa'atusatusa i le enzyme masani.Fa'aliliuga fa'asalalau fa'atasi ua uma ona fuaina, mo mea uma e lua o le fluorogenic ma oloa uma e lua, e fa'aogaina ai le felefele fa'amaopoopo spectroscopy.Fa'aliliuga fa'asalalau fa'atasi mo substrates fluorogenic ma le enzymatic hydrolysis oloa ua fuaina e tutusa, i le va o le 3.5-4.5 × 10−10 m2 s−1.O le mea lea e le o manino mai ai le fa'aleleia po'o le fa'ate'aina o le enzymatic kinetics ona o le eseesega i le fe'avea'i fa'aliliuga o mea'ai ma oloa.