BSA Cas: 9048-46-8 Fa'aisa-mamago pa'epa'e pa'epa'e Albumin
Numera Fa'amaumauga | XD90249 |
Igoa Oloa | Bovine Serum Albumin |
CAS | 9048-46-8 |
Fuafua mole | N/A |
Molecular Weight | N/A |
Fa'amatalaga Teuina | 2 i le 8°C |
Fa'atonu Fa'asologa o Fa'asologa | 35029070 |
Fa'amatalaga o oloa
Water | 5.0% maualuga |
Teuga | Taofi i se nofoaga malulu, matutu, pogisa |
Fa'aaliga | pa'epa'e pa'epa'e |
Aofa'iga Polotini (su'ega Biuret) | 98% min |
Ole mama ole BSA ile porotini(Electrophoresis test) | 96% min |
Solu (10 % ile H2O) | 15 |
pH (5% ile vai) | 6.5 - 7.4 |
OD403nm (1% ile H2O) | 0.15% maualuga |
Mo na'o su'esu'ega fa'aoga, ae le mo le fa'aogaina e tagata | fa'aoga su'esu'e, ae le mo le fa'aogaina e tagata |
Folasaga: O le BSA o se tasi o polotini e masani ona fa'aogaina i falesu'esu'e fa'aolaola, ma e ono le amana'ia lona taua i fa'ata'ita'iga ona e masani tele ma fa'aleaogaina.Bovine serum albumin (BSA), ua lauiloa foi o le vaega lona lima, o se globulin i le bovine serum o loʻo i ai 583 amino acid toega, ma le mamafa mole o le 66.430kDa ma le isoelectric point o le 4.7.O le BSA o lo'o i ai le tele o fa'aoga i su'esu'ega fa'aola kemikolo, e pei o le poloka poloka i poloka i sisifo.
Fa'aaogāga: Bovine Serum Albumin (BSA), e ta'ua fo'i o le vaega lona lima, o se globulin i totonu o le toto o povi, o lo'o i ai le 607 amino acid toega, ma e tele fo'i fa'aoga i su'esu'ega biochemical.Bovine serum albumin e masani ona faʻaaogaina e fai ma faʻamautu i le teuina o vaifofo ma tali tali o faʻatapulaʻaina enzymes poʻo enzymes faʻaleleia e faʻamautu ai le gaioiga o le enzyme ma puipuia ai le faʻaleagaina o le enzyme ma le adsorption e le faʻapitoa.
Galuega: BSA e masani ona faʻaaogaina e fai ma faʻamautu i le teuina o vaifofo ma tali faʻafefe o enzymes faʻatapulaʻa poʻo enzymes suia, ona o nisi enzymes e le mautu pe maualalo le gaioiga i le maualalo o faʻatonuga.A maeʻa ona faʻaopoopoina le BSA, e mafai ona faia le matafaioi o le "puipuiga" poʻo le "aveina", ma o le gaioiga o le tele o enzymes e mafai ona faʻaleleia atili pe a uma ona faʻaopoopo le BSA.Enzymes e le mana'omia le fa'aopoopoina o le BSA e masani ona le a'afia ile fa'aopoopoina ole BSA.Mo le tele o substrate DNA, e mafai e le BSCA ona fa'ato'a atoatoa le fa'amamago, ma e mafai ona ausia fa'asolosolo tipi.I le 37 ° C, pe a sili atu le faʻafefeteina o le gaogao i le 1 h, e mafai e le BSA ona faʻamautu le enzyme, aua i le tali faʻafefe e aunoa ma le BSA, e tele enzymes faʻasaina e mafai ona ola i le 37 ° C mo le 10 ~ 20min poʻo le taimi puupuu..I se fa'atusatusaga, e mafai e le BSA ona fusifusia ion u'amea ma isi vaila'au i totonu o le pa'u po'o le DNA substrate e fa'alavelaveina ai le gaioiga o endonucleases fa'asaina.
Fa'aoga: Standard grade bovine serum albumin (BSA, StandardGrade), lea e mafai ona fa'amalieina mana'oga o le tele o fa'ata'ita'iga masani, e pei o le immunoblocking agent, tissue cell (microbial manu ma iniseti sela, ma isi) aganuu meaʻai ma aganuu vaega, protein/enzyme stabilization reagents ma tulaga fa'atusatusaga o porotini.E mafai ona fa'afetauia le tele o mana'oga fa'ata'ita'i masani, e pei o le immunoblocking agent, protein/enzyme stabilizer, diluent, carrier and protein standard.E le gata i lea, e mafai foi ona faʻaaogaina mo immunoassays, aganuʻu cell ma faʻataʻitaʻiga hybridization e manaʻomia ai le maualuga o lagona.