Oloa

Gaoioiga olaola: BCIP (BCIP p-toluidine salt; X-phosphate p-toluidine salt) o se mea faʻapipiʻi chromogenic mo le suʻesuʻeina o lanu o le alkaline phosphatase.

Shiga toxins (Stxs) e gaosia e le enterohemorrhagic Escherichia coli (EHEC), lea e mafua ai faʻamaʻi pipisi o tagata ma e masani ona afaina ai.O laina o le Vero, e maua mai i fatuga'o manuki lanumeamata a Aferika, e fai ma sui o le tulaga auro mo le fuafuaina o aafiaga cytotoxic o Stxs.E ui lava i lo latou faʻaogaina i le lalolagi atoa, o le malamalama e uiga i fausaga tonu o le Stx receptor glycosphingolipids (GSLs) ma la latou faʻapotopotoga i lipid rafts e le lelei.O iinei matou te tuʻuina atu ai se suʻesuʻega faʻapitoa o Stx receptor GSLs ma lo latou tufatufaina atu i membranes-resistant detergent (DRMs), lea na saunia mai sela Vero-B4 ma faʻaaogaina e pei o lipid raft e tutusa.Na matou faailoaina le globotriaosylceramide (Gb3Cer) ma le globotetraosylceramide (Gb4Cer) o le GSL receptors mo Stx1a, Stx2a, ma Stx2e subtypes e faʻaaoga ai le TLC overlay detection faʻatasi ma MS.O le mea e le masani ai Stx receptor, globopentaosylceramide (Gb5Cer, Galβ3GalNAcβ3Galα4Galβ4Glcβ1Cer), lea na faʻamaonia faapitoa (faʻaopoopo i le Gb3Cer ma Gb4Cer) e Stx2e, na faʻatulagaina atoatoa.Lipoforms o Stx receptor GSLs na maua e fa'amautu ai vaega ceramide e aofia ai sphingosine (d18:1) ma le C24:0/C24:1 po'o le C16:0 fatty acid.E le gata i lea, o le faʻatasi ma faʻailoga o le lipid raft, SM ma le cholesterol, i DRMs fautuaina le fesoʻotaʻiga GSL ma microdomains membrane.O lenei suʻesuʻega e maua ai le faʻavae mo le suʻesuʻeina atili o le aʻafiaga o le lipid raft-sociated Stx receptors mo manuʻa oona o Vero-B4 sela.