Oloa

Ole mea fou ole Trinder's reagents ole vai-soluble aniline derivatives o lo'o fa'aaogaina lautele i su'esu'ega fa'ama'i ma su'ega biochemical.E tele fa'amanuiaga nai lo reagents chromogenic masani ile fa'ai'uga lanu ole gaioiga ole hydrogen peroxide.Ole mea fou ole Trinder's reagents e lava mautu e fa'aoga ile vaifofo ma faiga fa'ata'ita'iga ole su'esu'eina ole paipa.I le i ai o le hydrogen peroxide ma le peroxidase, o le tala a Trinder's reagent na faʻaalia e tali atu i le 4- aminoantipyrine (4-AA) poʻo le 3- methylbenzothiazole sulfonehydrazone (MBTH) i le taimi o le oxidative coupling reaction.Fausia ni lanu viole ma le lanumoana.O le molar absorbance o le vali faʻatasi ma le MBTH e 1.5- 2 taimi sili atu nai lo le vali faʻatasi ma le 4-AA;ae ui i lea, o le 4-AA solution na sili atu ona mautu nai lo le MBTH solution.O le mea'ai e fa'ama'i fa'ama'iina e lona oxidase e maua ai le hydrogen peroxyide.Ole fa'aogaina ole hydrogen peroxyide e fetaui ma le fa'aogaina ole substrate.O le mea lea, o le aofaʻi o substrate e mafai ona faʻamoemoeina e ala i le atinaʻeina o lanu o le faʻaogaina o le faʻaogaina o le oxidative.O le kulukose, ava malosi, acyl-CoA ma le cholesterol e mafai ona faʻaogaina e suʻe ai na mea faʻapipiʻi faʻatasi ma le tala Trinder's reagent ma le 4-AA.E 10 mea fou a Trinder's reagents o lo'o avanoa.I totonu o le Trinder's reagents fou, TOOS e masani ona faʻaaogaina.Ae ui i lea, mo se mea faʻapitoa, suʻega vasega eseese o tala fou a Trinder's reagents e manaʻomia e atiaʻe ai le faiga sili ona lelei.