4-Nitrophenyl-alpha-L-fucopyranoside CAS:10231-84-2 Pa'epa'e i le lanu samasama samasama pa'u tioata.
Numera Fa'amaumauga | XD90253 |
Igoa Oloa | 4-Nitrophenyl-alpha-L-fucopyranoside |
CAS | 10231-84-2 |
Fuafua mole | C12H15NO7 |
Molecular Weight | 285.25 |
Fa'amatalaga Teuina | 2 i le 8 °C |
Fa'atonu Fa'asologa o Fa'asologa | 29400000 |
Fa'amatalaga o oloa
Solubility | Fofo manino, leai se lanu |
Su'ega | 99% |
Fa'aaliga | Pa'epa'e i le samasama samasama pauta tioata |
TLC | Nofoaga tasi |
Mamaa HPLC | Min 98% |
Ole auala e tu'ufa'atasia ai a'afiaga o suiga fa'atasi e suia ai le gaioioiga enzymatic e le faigofie ona va'aia ona o nei a'afiaga e le'o fa'aopoopo i taimi uma i se laina laina.O le mea lea, o le faʻamalamalamaina o aʻafiaga o suiga faʻatasi o toega amino acid e fusifusia ai le substrate e mafai ona faia se sao taua i le malamalama i le tulaga faʻapitoa o enzymes.I le beta-glycosidase mai Spodoptera frugiperda (Sfbetagly), o totoe uma Q39 ma le E451 fegalegaleai ma le substrate ma e taua tele mo le faʻamalamalamaina o mea faʻapitoa.O suiga fa'alua a Sfbetagly (A451E39, S451E39 ma S451N39) na saunia e mutagenesis fa'atonu i luga ole laiga, fa'aalia ile siama ma fa'amamāina ile fa'aogaina ole affinity chromatography.O nei enzymes na faʻaalia e faʻaaoga ai le p-nitrophenyl beta-galactoside ma le p-nitrophenyl beta-fucoside e fai ma sui.O le k cat/Km ratio mo mutants tasi ma le lua o Sfbetagly o loʻo i ai suiga faʻatonutonu nofoaga i tulaga Q39 ma E451 na faʻaaogaina e faʻaalia ai le aʻafiaga o le malosi saoloto o le ESdouble dagger (enzyme-transition state complex) o suiga faʻalua (Gdouble). daggerxy) e le o le aofaʻi o aʻafiaga e mafua mai i suiga e tasi (Gdouble daggerx ma Gdouble daggery).O lenei eseesega i le Gdouble dagger o loʻo faʻaalia ai o aʻafiaga o suiga e tasi o loʻo faʻapipiʻi vaega.O lea la, o lenei aafiaga masani e na'o le tasi e faitau i le Gdouble daggerxy.O fa'amaumauga fa'amalama i luga o beta-glycosidases e fa'aalia ai le iai o se fa'amauga hydrogen bidentate e aofia ai toega Q39 ma le E451 ma le kulupu hydroxyl tutusa o le substrate.O le mea lea, o fa'amaumauga e lua o le thermodynamic ma le crystallographic o lo'o fautua mai ai o toega Q39 ma le E451 e fa'atino se fa'afeagai i luga oa latou fegalegaleaiga faʻatasi ma le substrate.