Oloa

4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) o se mea faʻapipiʻi faʻaogaina o le 4-nitrophenyl (pNP) glycopyranoside mo le suʻeina o gaioiga α-galactosidase.O le aofaʻi o le pNP na tuʻuina atu na matua faʻateleina pe a faʻaaogaina le 4-Nitrophenyl α-D-galactopyranoside e fai ma sui.

O le fa'aaogaina o le 4-nitorophenol (pNP) -glycopyranosidespseudosubstrates, o le aofa'i o pNP fa'asaolotoina mai le4-Nitrophenylα-D-galactopyranoside e matua'i maualuga atu nai lo le isi NP-glycopyranosidesatpH4.0ae leai se gaoioiga na su'esu'eina ilepH7. pyranosidesasa mea fa'apalapala fa'aalia o lo'o fa'asa'oloto le galactoseresidues mai le 4-Nitrophenylα-D-galactopyranoside tele i lepH4.0, iethepHlevelpe'o le mea e maua ai le fa'aitiitiga i le tele.

Laega ole α-D-galactosidase (α-D-Galactosidase).E fusifusia i le E. coli lactose protein carrier.Medium mo le α-D-galactose.

O se auala vave mo le suʻesuʻeina o le gaioiga o vailaʻau faʻamaʻi e faasaga i le fefete ma le gaʻo ua atiaʻe.O le suʻega e faʻavae i luga o le tuʻuina atu o le enzyme intracellular, maltase (alpha-glucosidase).O le gaioiga maltase faʻamalolo na fuaina lanu e ala i le gaosiga o le p-nitrophenol mai le p-nitrophenyl-alpha-D-glucopyranoside (PNPG).O le gaioioiga lytic o fa'aputuga fa'aantimicrobial 'ese'ese na fuaina fa'asaga i sela fefete po'o spores o lo'o fa'atupuina ole sigi filamentous.Lytic anti-fefete gaoioiga e mafai ona iloa i totonu o le 20 min incubation i le 30 tikeri C faasaga i Saccharomyces cerevisiae, Candida albicans, ma Cryptococcus neoformans.Lytic anti-fungal gaoioiga na aliali mai ina ua mavae 2 h o incubation i 30 tikeri C faasaga germinating spore o Aspergillus niger ma Botrytis cinerea.O sela atoa o so'o se fefete po'o sigi e le'i fa'asalaina lava PNPG i totonu ole 3 itula ile 30 tikeri C e maua ai se suiga lanu e iloagofie.Ole gaioioiga lytic ole enzymes (fa'ata'ita'iga, Lyticase), vaila'au fa'ama'i (fa'ata'ita'iga, Amphotericin B), ma se siama e gaosia ai vaila'au fa'ama'i na maua ile su'ega.O le su'esu'ega fa'afefete fa'afefete ua fa'afetaui i se fa'asologa 96-well microtiter.O su'esu'ega uma e lua na maua ai se su'esu'ega vave, ma'ale'ale, ma toe fa'afo'iina o le fa'afefete fa'afefete ma le fa'agaioiga fa'asa.