3- [(3- Cholanidopropyl) dimethylammonio] -1 -propanesulfonate Cas: 75621-03-3 98%
Numera Fa'amaumauga | XD90075 |
Igoa Oloa | 3-[(3-Cholanidopropyl)dimethylammonio]-1-propanesulfonate |
CAS | 75621-03-3 |
Fuafua mole | C32H58N2O7S |
Molecular Weight | 614.88 |
Fa'amatalaga Teuina | Ambient |
Fa'atasi Tulafono Fa'asologa | 29242970 |
Fa'amatalaga o oloa
IR | Faatusatusa i le faasinomaga |
Ma'imau ile Fa'amago | <2% |
TLC | Tasi le nofoaga |
Nitrogen | 4.46 - 4.65 |
Fa'aaliga | Pa'u pa'epa'e |
Solu (10 % ile H2O) | E manino ma leai se lanu |
Amioga (0.1M ile H2O) | <10us/cm |
Su'ega HPLC | >98% |
UV (5% ile H2O) A290nm | <0.5 |
UV (5% ile H2O) A400nm | <0.02 |
Solvent Content (e GC) Dimethylformamide | <2.0% |
3-[3-(cholamidopropyl) dimethylamino] propanesulfonic acid fa'aoga masima i totonu ma le fa'aogaina o auala
Gaoioiga Fa'alesoifua maloloina: CHAPS, o se mea e maua mai i le Cholicacid, o se vaila'au zwitterionic e solubilizes membrane proteins.O lo'o fa'aogaina le CHAPS e fa'amautu ai le tele o palatini-DNA lavelave ma e mafai ona fa'atumauina le gaioiga fa'aolaola ole polotini ile vaifofo.
Fa'aoga: O le CHAPS ose vaila'au zwitterionic e le-denaturing mo le biochemistry membrane.E mafai ona fa'aogaina e fa'amalo ai polotini o le membrane ma fa'alavelaveina fegalegaleai polotini-porotini.O le CHAPS e maualalo le mamafa o le micellar molecular (6,150) ma le maualuga maualuga o le micelle concentration (6-10 mM) ma e mafai ona aveese mai le faʻataʻitaʻiga e ala ile toto.E talafeagai foi mo le solubilizing polotini i le isoelectric taulaʻi ma 2D electrophoresis.O le CHAPS e masani ona fa'aogaina ile fa'aoga ole isoelectric fa'ale-aganu'u (urea-free) ma ua maua e maua ai le fa'ai'uga sili ona lelei mo nisi o tapenaga i lalo ole sela ma polotini o la'au.E masani ona fa'aogaina fa'aputuga i le va o le 2-4% (w/v) mo gels e taula'i le isoelectric.
Fa'aaogāina: O se vaimea e le fa'a-denaturing zwitterionic mo membrane biochemistry, lea e mafai ona fa'aoga e solubilize proteins membrane ma fa'alavelaveina fegalegaleaiga porotini-porotini;talafeagai foi mo le solubilizing polotini i isoelectric taulaiga ma lua-dimensional electrophoresis;e masani ona fa'aogaina i le Non-denaturing (leai se urea) fa'aoga isoelectric;RNA ma DNA faʻapipiʻiina, leai se gaioiga RNase ma DNase
Fa'aaogāina: Amphoteric surfactant, CHAPS ose vaila'au zwitterionic e le-denaturing, protein lysate mo le solubilizing membrane porotini ma lysing fegalegaleaiga i le va o polotini ma polotini.