Oloa

O le polotini phosphorylation e faʻatonutonu ai le tele o galuega feaveaʻi ma e iai sona sao taua i le amataga o le atinaʻeina o laau.Ina ia faʻamaeʻaina ma faʻalauteleina i luga o suʻesuʻega talu ai o le protein phosphorylation i Arabidopsis seedlings sa matou faʻaaogaina se isi auala e tuʻufaʻatasia ai le faʻaogaina o le protein i lalo o tulaga e le faʻaleagaina ma le faʻamaoaigaina o le metal-ion affinity chromatography (IMAC) o phosphoproteins intact i Rubisco-depleted extracts, sosoo ai ma le faʻamaonia. fa'aogaina le lua-dimensional gel electrophoresis (2-DE) ma vai chromatography-tandem mass spectrometry (LC-MS/MS).I-gel trypsin digestion ma le auʻiliʻiliga o vaega gel filifilia na iloa ai 144 phosphorylated peptides ma toega, lea na o le 18 phosphopeptides ma le 8 phosphosite na maua i le PhosPhAt 4.0 ma le P3DB Arabidopsis thaliana phosphorylation site databases.E sili atu ma le afa o le 82 phosphoproteins faʻamaonia sa aʻafia i le metabolism carbohydrate, photosynthesis/respiration poʻo le oxidative stress response mechani sms.O le faʻatamaoaigaina o phosphoproteins faʻamaʻi aʻo leʻi oʻo i le 2-DE ma le LC-MS / MS e foliga mai e faʻaleleia ai le suʻesuʻeina o le phosphorylated threonine ma tyrosine toega pe a faʻatusatusa i metotia e faʻaogaina ai le faʻatamaoaigaina o le peptide, e faʻapea o auala e lua e fai si faʻatasi i tulaga o le faʻaogaina o nofoaga o le phosphorylation.O le fa'atusatusaina o fa'ai'uga mo tama'i la'au ma mea na maua muamua mo lau'au Arabidopsis matutua ua fa'ailoa mai ai polotini e lima e ese'ese le phosphorylated i totonu o nei masini, e fa'aalia ai le gafatia o lenei metotia mo le su'esu'eina o le malosi o le protein phosphorylation i le taimi o le atina'eina o laau.