Oloa

Ole faʻamaʻi gaʻo gaʻo ole ava (NAFLD) o loʻo i ai le tele o faʻamaʻi faʻamaʻi e amata mai i le steatosis vaivai e faʻaalia i le taofiofia o lipids i totonu ole ate i le steatohepatitis (NASH) e faʻaalia ai le faʻaputuina o gaʻo, mumū, paluni ma le faʻaleagaina o hepatocytes, ma fibrosis.Mulimuli ane, o le steatohepatitis e mafai ona iu i le ate cirrhosis ma le hepatocellular carcinoma. I lenei suʻesuʻega ua matou suʻesuʻeina ni faʻalavelave eseese e tolu, A / J, C57BL / 6J, ma le PWD / PhJ, e faʻaalia ai tikeri eseese o le steatohepatitis pe a faʻaaogaina se 3,5-diethoxycarbonyl. -1,4-dihydrocollidine (DDC) o loʻo iai meaʻai.RNA-Seq suʻesuʻega faʻamatalaga faʻamatalaga, suʻesuʻega o porotini ma faʻataʻitaʻiga faʻasolosolo na faʻaaogaina e faʻamaonia ai kenera / porotini ma faʻalavelave faʻalavelave o faʻataʻitaʻiga ate ate ile DDC-togafitiga.Ole su'esu'ega ole auala na fa'aalia ai suiga ole arachidonic acid (AA) ma le S-adenosylmethionine (SAMe) metabolism i luga o isi auala.Ina ia malamalama i suiga metabolic o le arachidonic acid metabolism i le malamalama o faʻamatalaga faʻamatalaga faʻamaʻi o se faʻataʻitaʻiga kinetic o lenei auala na atiaʻe ma faʻaleleia e tusa ai ma tulaga metabolite.Mulimuli ane, na faʻaaogaina le faʻataʻitaʻiga e suʻesuʻe ai i le silico aʻafiaga o vailaʻau faʻapitoa mo le steatohepatitis.Na matou faʻaalia le AA / eicosanoid metabolism e matua faʻalavelaveina i isumu DDC e faʻaaogaina ai le tuʻufaʻatasia o se faʻataʻitaʻiga ma le silico approach.O la matou suʻesuʻega o le AA / eicosanoid metabolic pathway o loʻo taʻu mai ai o le 5-hydroxyeicosatetraenoic acid (5-HETE), 15-hydroxyeicosatetraenoic acid (15-HETE) ma le prostaglandin D2 (PGD2) o loʻo faʻalavelave i isumu DDC.Matou te faʻaalia atili o se faʻataʻitaʻiga malosi e mafai ona faʻaaogaina mo le faʻamaonia o le vaʻaiga o suiga faʻasolosolo e faʻavae i luga o faʻamaumauga transcriptomics i totonu o se faʻamaʻi faʻamaʻi.E le gata i lea, o le SAMe metabolism na iloa o loʻo faʻalavelave ona o togafitiga DDC.Ole tele o kenera ma nisi metabolites o lenei module e fa'aalia ai le eseesega i le va o le A/J ma le C57BL/6J i le tasi itu ma le PWD/PhJ i le isi itu.